Microbiologist · Scientist · Developer

Mark
Zhang

At the intersection of microbiology, biopharmaceuticals, and software engineering. From stomach cancer research to HPLC analytics to digital pathology — bridging the bench and the terminal.

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// professional experience

Where I've worked.

Stomach Cancer Research Lab

Microbiology Researcher

Investigated the role of H. pylori and the gastric microbiome in stomach cancer pathogenesis. Performed bacterial culture, DNA extraction, PCR amplification, and microbiome sequencing analysis to characterise tumour-associated microbial communities.

Cell Culture PCR 16S rRNA Aseptic Technique H. pylori
0 25 50 75 100 Retention Time (min) mAU 3.2 min 7.8 min
PPD (now Thermo Fisher Scientific)

Biopharmaceutical Analyst

Performed bioanalytical testing on drug candidates using HPLC and LC-MS/MS methods under GLP-compliant conditions. Executed method validation, sample preparation, and data analysis in support of pre-clinical and clinical pharmacokinetic studies.

HPLC LC-MS/MS GLP Method Validation Empower
H&E
Tempus AI

Histology Technician

Processed clinical tissue specimens for molecular and pathology workflows supporting precision medicine. Performed sectioning, H&E staining, immunohistochemistry, and quality review of slides destined for genomic sequencing and AI-driven diagnostics.

Microtomy H&E Staining IHC FFPE Processing Digital Path

Bench science meets
software.

I'm a scientist and developer with hands-on experience across microbiology, bioanalytical chemistry, and histopathology — combined with a genuine passion for building software that makes laboratory and research workflows more efficient.

My career has taken me from characterising the gastric microbiome in stomach cancer specimens, to running GLP-compliant HPLC assays on drug candidates at PPD (now Thermo Fisher), to preparing and reviewing clinical tissue samples for AI-driven diagnostics at Tempus.

When I'm not at the bench or the keyboard, I'm exploring the intersection of life-science data and code — building tools, automating pipelines, and looking for the next interesting problem to solve.

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Industry positions
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Samples processed
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Lab disciplines
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Programming languages

// skills & tools

What I work with.

A hands-on toolkit built across microbiology research, GLP bioanalytical labs, digital pathology, and full-stack development. The focus is on roles where regulated science and software meet.

GMP / GLP Compliance
Core
Worked within 21 CFR Part 11 bioanalytical workflows at PPD, including batch records, deviation handling, and audit readiness.
QC Analyst LIMS
SOP Authoring & Execution
Core
Authored and executed SOPs supporting GLP sample analysis at PPD. A foundational requirement across regulated lab roles.
QC Analyst Histotech
Quality Assurance & Auditing
Proficient
Contributed to internal QA audits and CAPA follow-through at PPD, a standard requirement on QC Analyst job postings.
QC Analyst
Chain of Custody & Accessioning
Core
Handled clinical specimen intake and chain-of-custody documentation at Tempus AI, the entry point for every LIMS and pathology pipeline.
Histotech LIMS
Aseptic Technique
Core
Sterilization, autoclave operation, and BSL-2 aseptic work. Baseline competency for microbiology QC and sterile pharmaceutical environments.
QC Analyst
HPLC
Core
Ran and maintained HPLC methods for drug metabolite quantification at PPD under GLP conditions. A primary requirement for QC Analytical Chemist roles.
QC Analyst
Protein Assays & SDS-PAGE
Proficient
Quantitative protein workflows and gel-based separation techniques, forming part of the standard toolkit for biologics QC release testing.
QC Analyst
PCR & DNA Sequencing
Proficient
PCR amplification and downstream sequencing used for 16S microbiome profiling, with direct relevance to molecular QC and clinical sequencing labs.
QC Analyst LIMS
Immunofluorescence
Proficient
Multi-channel IF staining and imaging, bridging molecular assays with image-based digital pathology QC.
Histotech
H&E Staining
Core
Routine and special stains run on the Tissue-Tek Prisma at Tempus AI. A foundational skill for HT and HTL positions.
Histotech
Tissue-Tek Prisma
Core
Daily operation and troubleshooting of the Prisma auto-stainer platform at Tempus AI. Named-instrument experience that recruiters search for directly.
Histotech
Dreampath Fina
Proficient
Coverslipper workflow at Tempus AI. Another named-instrument line item frequently listed on digital pathology postings.
Histotech
Cryostat & Microtomy
Proficient
Frozen sectioning and block preparation feeding downstream staining and digital imaging pipelines. Core content on the ASCP HTL exam.
Histotech
LIMS
Core
Daily LIMS use at Tempus AI for specimen tracking, accessioning, and result entry. A direct on-ramp to LIMS Analyst positions.
LIMS
SQL
Proficient
Relational database modeling and query authoring. Often the deciding skill that moves a lab tech into LIMS Analyst consideration.
LIMS
JavaScript / React / Git
Proficient
Full-stack projects including Movie Bot and CTA Transit Tracker, demonstrating that the science-plus-code background is backed by real shipped work.
LIMS
// credentials
ASCP HTL Exam planned
BSc Molecular & Cellular Biology UIUC

// projects

Selected work.

Microbiology

Gastric Microbiome Study

Characterized microbial community composition in stomach cancer biopsy specimens using 16S rRNA sequencing and bioinformatics analysis to identify potential pathogenic signatures.

Biopharma

HPLC Method Development

Developed and validated HPLC methods for quantifying drug metabolites in biological matrices under GLP conditions, supporting clinical pharmacokinetic data submissions.

Histology

Clinical Tissue QC Pipeline

Contributed to tissue processing and quality review workflows at Tempus, ensuring slide quality met thresholds for downstream genomic sequencing and AI-based pathology analysis.

Software

Movie Bot

Discord bot built with Node.js and discord.js that searches the TMDB API and returns interactive poster collage embeds with button-based navigation.

Software

CTA Transit Tracker

Cross-platform Expo app that maps live CTA buses and 'L' trains in real time, with Turf.js dead-reckoning interpolation and a cached Node/Express proxy in front of the CTA APIs.

Research

H. pylori Virulence Profiling

Profiled CagA and VacA genotypes across clinical H. pylori isolates to correlate virulence factor expression with disease severity and tissue pathology findings.

Analytical

Bioanalytical Data Automation

Built Python scripts to automate sample tracking, data reformatting, and report generation from Empower chromatography outputs, reducing manual processing time.

Let's connect.

Open to laboratory scientist roles, research collaborations, and opportunities where bench science meets software. Drop me a message — I try to respond within 48 hours.